Assessing nuclear versus mitochondrial cell-free DNA (cfDNA) by qRT-PCR and droplet digital PCR using a piglet model of perinatal asphyxia.

Marie BitencBenedicte Grebstad TuneMaria MelheimMonica Atneosen-ÅseggXiaoran LaiPolona RajarRønnaug SolbergLars Oliver Baumbusch

Published in: Molecular biology reports (2022)

Both, qRT-PCR and the droplet digital PCR technique identified overall similar patterns for the concentration changes of cfDNA; but, the more sensitive digital PCR methodology might be required to identify minimal responses.

Keyphrases

real time pcr
high throughput
single cell
oxidative stress
pregnant women