Diagnosis of Apis dorsata venom allergy: use of recombinant allergens of Apis mellifera and a passive basophil activation test.

Peshala GunasekaraS M HandunnettiSunil PremawansaE W R A WitharanaIndra P RatnayakePradeep KaluarachchiChandima KarunatilakeR K S DiasG A S PremakumaraW M D K DasanayakeSuranjith L SeneviratneRajiva de Silva

Published in: Clinical and molecular allergy : CMA (2022)

rApi m 1 and 10 when combined was sensitive for the diagnosis of A. dorsata allergy. This combination had the lowest cross-reactivity rate with Vespula vulgaris. The passive BAT is highly sensitive in A. dorsata allergy. The basophil reactivity was significantly higher in severe anaphylaxis compared to mild/moderate anaphylaxis. This finding should be further explored in further studies.

Keyphrases

tandem mass spectrometry
simultaneous determination
atopic dermatitis
infectious diseases
early onset
high intensity
fluorescent probe
drug induced
cell free
label free