Stability of Biomimetically Functionalised Alginate Microspheres as 3D Support in Cell Cultures.
María Inmaculada García-BriegaJoaquín Ródenas-RochinaLuis Amaro MartinsSenendxu Lanceiros-MéndezGloria Gallego-FerrerAmparo SempereJosé Luís Gómez RibellesPublished in: Polymers (2022)
Alginate hydrogels can be used to develop a three-dimensional environment in which various cell types can be grown. Cross-linking the alginate chains using reversible ionic bonds opens up great possibilities for the encapsulation and subsequent release of cells or drugs. However, alginate also has a drawback in that its structure is not very stable in a culture medium with cellular activity. This work explored the stability of alginate microspheres functionalised by grafting specific biomolecules onto their surface to form microgels in which biomimetic microspheres surrounded the cells in the culture, reproducing the natural microenvironment. A study was made of the stability of the microgel in different typical culture media and the formation of polyelectrolyte multilayers containing polylysine and heparin. Multiple myeloma cell proliferation in the culture was tested in a bioreactor under gentle agitation.
Keyphrases
- tissue engineering
- induced apoptosis
- wound healing
- cell proliferation
- cell cycle arrest
- single cell
- multiple myeloma
- cell therapy
- stem cells
- molecularly imprinted
- endoplasmic reticulum stress
- venous thromboembolism
- oxidative stress
- signaling pathway
- pi k akt
- mass spectrometry
- mesenchymal stem cells
- extracellular matrix
- ionic liquid
- liquid chromatography