Bioactivity Potential of Bioceramic-Based Root Canal Sealers: A Scoping Review.
Mauro Schmitz EstivaletLucas Peixoto de AraújoFelipe ImmichAdriana Fernandes da SilvaNadia de Souza FerreiraWellington Luiz de Oliveira da RosaEvandro PivaPublished in: Life (Basel, Switzerland) (2022)
According to the methodology used, the most prevalent methods to assess bioactivity in acellular form were the immersion of the material in Hank's balanced salt solution, followed by surface observation with scanning electron microscopy and energy dispersive X-ray. In cell cultures, the chosen method was usually Alizarin Red staining, followed by the evaluation of alkaline phosphatase enzymatic activity and the use of molecular biology tests.