Recognition and sensitive detection of CTCs using a controllable label-free electrochemical cytosensor.
Hong-Fen ZhangFangmiao LiangXuemei WuYang LiuAnjia ChenPublished in: Mikrochimica acta (2020)
An excellent atomic layer deposition (ALD) method was adopted for the controllable systhesis of a xFe2O3-nPt (or nPt-xFe2O3)-coated graphene nanostructure (xFe2O3-nPt@graphene). The produced nanomaterials have been characterized by transmission electron microscopy (TEM), cyclic voltammetry (CV), and X-ray photoelectron spectroscopy (XPS). It is shown that xFe2O3 and nPt were effectively tailored and deposited on the graphene. A simple, rapid, and sensitive electrochemical cytosensor based on the controllable nanomaterials was successfully developed for MCF-7 cells detection by combining the high affinity and specificity of an aptamer. The prepared cytosensor displays a linear response to MCF-7 in the concentration range 18 to 1.5 × 106 cell mL-1 with the detection limit of 6 cell mL-1 (at an S/N of 3). This cytosensor was applied to detect circulating tumor cells (CTCs) in patient blood and the results were satisfied. The experimental results indicate that the proposed controllable electrochemical cytosensor is highly-sensitive, and convenient for clinical detection of breast CTCs. Graphical abstract.
Keyphrases
- label free
- circulating tumor cells
- sensitive detection
- electron microscopy
- loop mediated isothermal amplification
- single cell
- circulating tumor
- cell therapy
- high resolution
- breast cancer cells
- induced apoptosis
- quantum dots
- carbon nanotubes
- walled carbon nanotubes
- computed tomography
- cell proliferation
- smoking cessation
- magnetic resonance
- mesenchymal stem cells
- oxidative stress
- bone marrow
- cell cycle arrest
- endoplasmic reticulum stress
- cell death
- real time pcr
- cell free