Directed evolution of the PcaV allosteric transcription factor to generate a biosensor for aromatic aldehydes.
Leopoldo F M MachadoAndrew CurrinNeil DixonPublished in: Journal of biological engineering (2019)
This is the first study to report directed evolution of a member of the MarR aTF family, and demonstrates the plasticity of the PCA biosensor by altering its ligand specificity to generate a biosensor for aromatic aldehydes.