Electronic DNA Analysis of CSF Cell-free Tumor DNA to Quantify Multi-gene Molecular Response in Pediatric High-grade Glioma.
Amy K BruzekKarthik RaviAshwath MuruganandJack WaddenClarissa May BabilaEvan CantorLeo TunkleKyle WierzbickiStefanie StallardRobert P DicksonIan WolfeRajen ModyJonathan SchwartzAndrea T FlynnPatricia L RobertsonKarin M MuraszkoCormac O MaherHugh J L GartonTingtin QinCarl KoschmannPublished in: Clinical cancer research : an official journal of the American Association for Cancer Research (2020)
Nanopore sequencing of ultra-short pHGG CSF cf-tDNA fragments is feasible, efficient, and sensitive with low-input samples thus overcoming many of the barriers restricting wider use of CSF cf-tDNA diagnosis and monitoring in this patient population.