Phenotypic and Genomic Characterization of Pseudomonas wuhanensis sp. nov., a Novel Species with Promising Features as a Potential Plant Growth-Promoting and Biocontrol Agent.

Plant growth-promoting rhizobacterial strain FP607 T was isolated from the rhizosphere of beets in Wuhan, China. Strain FP607 T exhibited significant antagonism toward several phytopathogenic bacteria, indicating that FP607 T may produce antimicrobial metabolites and has a stronger biocontrol efficacy against plant pathogens. Growth-promoting tests showed that FP607 T produced indole-3-acetic acid (IAA), NH 3 , and ferritin. The genome sequence of strain FP607 T was 6,590,972 bp long with 59.0% G + C content. The optimum temperature range was 25-30 °C, and the optimum pH was 7. The cells of strain FP607 T were Gram-negative, short, and rod-shaped, with polar flagella. The colonies on the King's B (KB) agar plates were light yellow, smooth, and circular, with regular edges. A phylogenetic analysis of the 16S rRNA sequence and a multilocus sequence analysis (MLSA) showed that strain FP607 T was most closely related to the type of strain Pseudomonas farris SWRI79 T . Based on a polyphasic taxonomic approach, strain FP607 T was identified as a novel species within the genus Pseudomonas , for which the name Pseudomonas wuhanensis sp. nov. was proposed. The type of strain used was FP607 T (JCM 35688, CGMCC 27743, and ACCC 62446).