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Quantification of DNA methylation and hydroxymethylation in Alzheimer's disease mouse model using LC-MS/MS.

Qionglin HuangSimin XuMingming MoQingjin YangJian LiYu ZhongJunjie ZhangLijian ZhangXiaoxiao YeZhou LiuChun Cai
Published in: Journal of mass spectrometry : JMS (2019)
Ambiguous alteration patterns of 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC) involved in Alzheimer's disease (AD) obstructed the mechanism investigation of this neurological disorder from epigenetic view. Here, we applied a fully quantitative and validated LC-MS/MS method to determine genomic 5mC and 5hmC in the brain cortex of 3 month-aged (12, 15, and 18 month) AD model mouse and found significant increases of 5mC and 5hmC levels in different months of AD mouse when compared with age-matched wild-type control and exhibited rising trend from 12-month to 18-month AD mouse, thereby supporting genomic DNA methylation and hydroxymethylation were positively correlated with developing AD.
Keyphrases
  • dna methylation
  • mouse model
  • gene expression
  • wild type
  • genome wide
  • copy number
  • cognitive decline
  • functional connectivity
  • white matter
  • resting state
  • brain injury