Saccharomyces cerevisiae Shuttle vectors.
Robert GnüggeFabian RudolfPublished in: Yeast (Chichester, England) (2017)
Yeast shuttle vectors are indispensable tools in yeast research. They enable cloning of defined DNA sequences in Escherichia coli and their direct transfer into Saccharomyces cerevisiae cells. There are three types of commonly used yeast shuttle vectors: centromeric plasmids, episomal plasmids and integrating plasmids. In this review, we discuss the different plasmid systems and their characteristic features. We focus on their segregational stability and copy number and indicate how to modify these properties. Copyright © 2017 John Wiley & Sons, Ltd.
Keyphrases
- saccharomyces cerevisiae
- escherichia coli
- copy number
- mitochondrial dna
- klebsiella pneumoniae
- gene therapy
- induced apoptosis
- genome wide
- biofilm formation
- cell cycle arrest
- dna methylation
- circulating tumor
- single molecule
- gene expression
- cell free
- signaling pathway
- cell proliferation
- multidrug resistant
- cell death
- endoplasmic reticulum stress
- nucleic acid
- cystic fibrosis
- circulating tumor cells
- candida albicans