Detection of Myosin 1g Overexpression in Pediatric Leukemia by Novel Monoclonal Antibodies.
Rosa Isela Rodríguez-TéllezRosa María Ribas-AparicioGenaro Patiño LópezPublished in: International journal of molecular sciences (2022)
Myosin 1g (Myo1g) is a mechanoenzyme associated with actin filaments, expressed exclusively in hematopoietic cells, and involved in various cellular functions, including cell migration, adhesion, and membrane trafficking. Despite the importance of Myo1g in distinct functions, there is currently no monoclonal antibody (mAb) against Myo1g. mAbs are helpful tools for the detection of specific antigens in tumor cells and other tissues. The development of mAbs against targeted dysregulated molecules in cancer cells remains a crucial tool for aiding in the diagnosis and the treatment of patients. Using hybridoma technology, we generated a panel of hybridomas specific for Myo1g. ELISA, immunofluorescence, and Western blot assay results revealed the recognition of Myo1g by these novel monoclonal antibodies in normal and transformed T and B cells. Here, we report the development and application of new monoclonal antibodies against Myo1g for their potential use to detect its overexpression in acute lymphoblastic leukemia (ALL) patients.
Keyphrases
- cell migration
- monoclonal antibody
- acute lymphoblastic leukemia
- end stage renal disease
- bone marrow
- ejection fraction
- induced apoptosis
- chronic kidney disease
- newly diagnosed
- transcription factor
- binding protein
- loop mediated isothermal amplification
- acute myeloid leukemia
- real time pcr
- high throughput
- south africa
- single cell
- dendritic cells
- cell cycle arrest
- prognostic factors
- label free
- risk assessment
- signaling pathway
- cell death
- oxidative stress
- drug delivery
- cystic fibrosis
- childhood cancer
- sensitive detection