Spatially resolved single-cell translatomics at molecular resolution.
Hu ZengJiahao HuangJingyi RenConnie Kangni WangZefang TangHaowen ZhouYiming ZhouHailing ShiAbhishek AdithamXin SuiHongyu ChenJennifer A LoXiao WangPublished in: Science (New York, N.Y.) (2023)
The precise control of messenger RNA (mRNA) translation is a crucial step in posttranscriptional gene regulation of cellular physiology. However, it remains a challenge to systematically study mRNA translation at the transcriptomic scale with spatial and single-cell resolution. Here, we report the development of ribosome-bound mRNA mapping (RIBOmap), a highly multiplexed three-dimensional in situ profiling method to detect cellular translatome. RIBOmap profiling of 981 genes in HeLa cells revealed cell cycle-dependent translational control and colocalized translation of functional gene modules. We mapped 5413 genes in mouse brain tissues, yielding spatially resolved single-cell translatomic profiles for 119,173 cells and revealing cell type-specific and brain region-specific translational regulation, including translation remodeling during oligodendrocyte maturation. Our method detected widespread patterns of localized translation in neuronal and glial cells in intact brain tissue networks.
Keyphrases
- single cell
- rna seq
- induced apoptosis
- cell cycle
- cell cycle arrest
- high throughput
- genome wide
- cell proliferation
- endoplasmic reticulum stress
- multiple sclerosis
- oxidative stress
- cell death
- single molecule
- cerebral ischemia
- pi k akt
- dna methylation
- high resolution
- copy number
- neuropathic pain
- blood brain barrier
- resting state
- spinal cord
- subarachnoid hemorrhage
- bioinformatics analysis