Mismatch binding ligand upregulated back-splicing reaction producing circular RNA in a cellular model.

Lu Ni Takeshi Yamada Asako Murata Kazuhiko Nakatani

Published in: Chemical communications (Cambridge, England) (2022)

Circular RNA (circRNA) is a covalently closed single-stranded RNA produced from pre-mRNAs via back-splicing reaction, an alternative form of splicing. Here, we show naphthyridine carbamate dimer (NCD) upregulating the production of a circRNA from a pre-mRNA containing NCD-binding site UGGAA/UGGAA in cells, demonstrating the feasibility of small-molecule mediated circRNA production.

Keyphrases

small molecule
binding protein
induced apoptosis
cell cycle arrest
nucleic acid
protein protein
cell death
oxidative stress
dna binding
electron transfer
cell proliferation
pi k akt