Anticandidal activity of Croton heliotropiifolius Kunth essential oil is enhanced by N-acetylcysteine and itraconazole.
Bruno Coêlho CavalcantiIslay Lima MagalhãesDaniel Sampaio RodriguesVitória Pessoa de Farias CabralAmanda Dias BarbosaLívia Gurgel do Amaral Valente SáLisandra Juvêncio da SilvaJoão Batista de Andrade NetoCecília Rocha da SilvaManoel Odorico de MoraesCláudio Costa Dos SantosHélio Vitoriano Nobre JúniorPublished in: Future microbiology (2024)
Aim: Evaluate the anticandidal effect of Croton heliotropiifolius Kunth essential oil and its interaction with azoles and N-acetylcysteine (NAC) against planktonic cells and biofilms. Materials & methods: Broth microdilution and checkerboard methods were used to evaluate the individual and combined activity with fluconazole and itraconazole (ITRA). The antibiofilm effect of the oil was assessed in 96-well plates alone and combined with ITRA and NAC, and cytotoxicity determined by MTT. Results: The oil inhibited all Candida species growth. The activity was enhanced when associated with ITRA and NAC for planktonic cells and biofilms in formation. The effective concentrations were lower than the toxic ones to V79 cells. Conclusion: C. heliotropiifolius Kunth essential oil is an anticandidal alternative, and can be associated with ITRA and NAC.