Selective human factor VIII activity measurement after analytical in-line purification.

Andrea EngelmaierGerald SchrenkManfred BillweinHerbert GritschChristoph ZlabingerAlfred Weber

Published in: Research and practice in thrombosis and haemostasis (2022)

Combining antibody-mediated specific capture of human FVIII and a chromogenic activity assay resulted in a selective and sensitive measurement of human FVIII with no interference by endogenous, nonhuman FVIII, manufacturing process additives, or an FVIII activity-mimicking antibody.

Keyphrases

endothelial cells
induced pluripotent stem cells
high throughput
mass spectrometry
ionic liquid
single cell