Thermosensitive alginate-gelatin-nitrogen-doped carbon dots scaffolds as potential injectable hydrogels for cartilage tissue engineering applications.
Mojgan GhanbariMasoud Salavati-NiasariFatemeh MohandesPublished in: RSC advances (2021)
Hybrid injectable and biodegradable hydrogels based on oxidized alginate/gelatin and containing nitrogen-doped carbon dots (NCDs) as a reinforcement have been fabricated and crosslinked by 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC)/ N -hydroxysuccinimide (NHS) as the chemical crosslinking agents in the hydrogel system. The idea of composite hydrogels relies on the assumption that they supply a microenvironment that is convenient for the exchange of nutrients via a porous structure and cell proliferation and have mechanical characteristics that approximately match natural tissue. The effect of the NCD content on the morphology structure, mechanical strength, swelling ratio, and biodegradation has been investigated. The results indicate that nanocomposite hydrogels containing a higher content of NCDs have smaller pore sizes and higher mechanical properties. The in vitro biodegradation and swelling behavior demonstrated that increasing the amount of NCDs up to 0.06% decreased the swelling ratio and weight loss of the hydrogels. The composite hydrogels are biocompatible, as verified by the MTT assay of MG-63 cells. The N-doped graphene quantum dots considerably affect degradation and interaction within the cells and hydrogels.
Keyphrases
- tissue engineering
- quantum dots
- hyaluronic acid
- induced apoptosis
- cell proliferation
- weight loss
- cell cycle arrest
- drug delivery
- high throughput
- type diabetes
- sensitive detection
- roux en y gastric bypass
- extracellular matrix
- mass spectrometry
- high resolution
- gold nanoparticles
- heavy metals
- risk assessment
- carbon nanotubes
- wound healing
- human health
- high speed
- tandem mass spectrometry