Functional Voltage-Gated Sodium Channels Are Present in the Human B Cell Membrane.

B cells express various ion channels, but the presence of voltage-gated sodium (Na V ) channels has not been confirmed in the plasma membrane yet. In this study, we have identified several Na V channels, which are expressed in the human B cell membrane, by electrophysiological and molecular biology methods. The sensitivity of the detected sodium current to tetrodotoxin was between the values published for TTX-sensitive and TTX-insensitive channels, which suggests the co-existence of multiple Na V 1 subtypes in the B cell membrane. This was confirmed by RT-qPCR results, which showed high expression of TTX-sensitive channels along with the lower expression of TTX-insensitive Na V 1 channels. The biophysical characteristics of the currents also supported the expression of multiple Na V channels. In addition, we investigated the potential functional role of Na V channels by membrane potential measurements. Removal of Na + from the extracellular solution caused a reversible hyperpolarization, supporting the role of Na V channels in shaping and maintaining the resting membrane potential. As this study was mainly limited to electrophysiological properties, we cannot exclude the possible non-canonical functions of these channels. This work concludes that the presence of voltage-gated sodium channels in the plasma membrane of human B cells should be recognized and accounted for in the future.