A Sequentially Activated Probe for Imaging of Superoxide Anion and Peroxynitrite in PC12 Cells under Oxidative Stress.
Ying LiJing CaoXue WuJunjie KouTingting FengRuixin ZhangChang XuFanpeng KongBo TangPublished in: Analytical chemistry (2024)
Superoxide anion (O 2 ·- ) and peroxynitrite (ONOO - ), two important oxidants under oxidative stress, coexist in complex cell and organism systems, playing crucial roles in various physiological and pathological processes, particularly in neurodegenerative diseases. Despite the absence of robust molecular tools capable of simultaneously visualizing O 2 ·- and ONOO - in biosystems, the relationship between these two species remains understudied. Herein, we present sequentially activated fluorescent probe, DHX-SP , which exhibits exceptional selectivity and sensitivity toward O 2 ·- and ONOO - . This probe enables precise imaging of these species in living PC12 cells under oxidative stress conditions using distinct fluorescence signal combinations. Furthermore, the probe DHX-SP has the ability to visualize changes in O 2 ·- and ONOO - levels during ferroptosis of PC12 cells and in the Parkinson's disease model. These findings establish a connection between the crosstalk of the phosphorus group of O 2 ·- and ONOO - in PC12 cells under oxidative stress.
Keyphrases
- living cells
- oxidative stress
- fluorescent probe
- single molecule
- dna damage
- ischemia reperfusion injury
- high resolution
- diabetic rats
- induced apoptosis
- quantum dots
- ionic liquid
- cell death
- single cell
- endoplasmic reticulum stress
- nitric oxide
- photodynamic therapy
- bone marrow
- structural basis
- heat shock protein
- heavy metals