Spontaneously Blinking Fluorescent Protein for Simple Single Laser Super-Resolution Live Cell Imaging.
Yoshiyuki AraiHiroki TakauchiYuhei OgamiSatsuki FujiwaraMasahiro NakanoTomoki MatsudaYoshiyuki AraiPublished in: ACS chemical biology (2018)
Super-resolution imaging techniques based on single molecule localization microscopy (SMLM) broke the diffraction limit of optical microscopy in living samples with the aid of photoswitchable fluorescent probes and intricate microscopy systems. Here, we developed a fluorescent protein, SPOON, which can be switched off by excitation light illumination and switched on by thermally induced dehydration, resulting in an apparent spontaneous blinking behavior. This unique property of SPOON provides a simple SMLM-based super-resolution imaging platform which requires only a single 488 nm laser.
Keyphrases
- single molecule
- high resolution
- living cells
- high speed
- atomic force microscopy
- label free
- quantum dots
- high throughput
- small molecule
- protein protein
- magnetic resonance
- computed tomography
- mass spectrometry
- drug induced
- binding protein
- oxidative stress
- fluorescence imaging
- magnetic resonance imaging
- single cell
- amino acid
- diffusion weighted imaging