Genomic Analysis of Klebsiella pneumoniae ST258 Strain Coproducing KPC-2 and CTX-M-14 Isolated from Poultry in the Brazilian Amazon Region.
Tiago Barcelos ValiattiRodrigo CayôFernanda Fernandes SantosFrancisco Ozório Bessa-NetoRamon Giovani Brandão SilvaRuanita VeigaMárcia de Nazaré Miranda BahiaLívia Maria Guimarães Dutra GuerraAntônio Carlos Campos PignatariCintya de Oliveira SouzaDanielle Murici BrasilienseAna Cristina Galesnull nullPublished in: Antibiotics (Basel, Switzerland) (2022)
This study aimed to characterize a Klebsiella pneumoniae strain (KP411) recovered from the stool samples of poultry ( Gallus gallus ) in the Brazilian Amazon Region. The whole-genome sequencing of KP411 revealed the presence of an important arsenal of antimicrobial resistance genes to β-lactams ( bla CTX-M-14 , bla TEM-1B , bla KPC-2 , bla SVH-11 ), aminoglycosides [ aph(3″)- Ib, aph(6)-Id, aph(3')-Ia ], sulfonamides ( sul1, sul2 ), quinolones ( oqxAB ), fosfomycin ( fosA KP ), and macrolides [ mph(A) ]. Furthermore, our analyses revealed that the KP411 strain belongs to the ST258 clonal lineage, which is one of the main epidemic clones responsible for the dissemination of KPC-2 worldwide. Our data suggest that food-producing animals may act as reservoirs of multidrug-resistant K. pneumoniae belonging to the ST258 clone, and, consequently, contribute to their dissemination to humans and the environment.
Keyphrases
- klebsiella pneumoniae
- antimicrobial resistance
- multidrug resistant
- gram negative
- escherichia coli
- drug resistant
- single cell
- acinetobacter baumannii
- genome wide
- climate change
- risk assessment
- dna methylation
- big data
- gene expression
- urinary tract infection
- mass spectrometry
- human health
- transcription factor
- pseudomonas aeruginosa
- respiratory tract
- genome wide identification