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Structural elucidation a complex galactosyl and glucosyl-rich pectin from the pericarp of immature fruits of Juglans mandshurica Maxim.

Xi-Zhe SunQing-Yu ZhangSi-Liang JiangRong-Jian ZhuJun-Hong ChaiJun LiangHai-Xue KuangYong-Gang Xia
Published in: Glycoconjugate journal (2024)
A glucosyl-rich pectin, JMMP-3 (M w , 2.572 × 10 4  g/mol, O-methyl % = 3.62%), was isolated and purified from the pericarp of the immature fruit of Juglans mandshurica Maxim. (QingLongYi). The structure of JMMP-3 was studied systematically by infrared spectroscopy, monosaccharide compositions, methylation analysis, partial acid hydrolysis, and 1/2D-NMR. The backbone of JMMP-3 possessed a smooth region (→  4 GalA 1  →) and a hairy region (→  4 GalA 1  →  2 Rha 1  →) with a molar ratio of 2: 5. The substitution of four characteristic side chains (R 1 -R 4 ) occurs at C-4 of → 2,4)-α-Rhap-(1→, where R 1 is composed of → 5)-α-Araf-(1→, R 2 is composed of → 4)-β-Galp-(1 → and β-Galp-(1→, R 3 is composed of α-Glcp-(1→, →4)-α-Glcp-(1 → and → 4,6)-α-Glcp-(1→, and R 4 is composed of → 5)-α-Araf-(1→, β-Galp-(1→, → 4)-β-Galp-(1→, → 3,4)-β-Galp-(1→, → 4,6)-β-Galp-(1 → and → 2,4)-β-Galp-(1 → . In addition, the antitumor activity of JMMP-3 on HepG2 cells was preliminarily investigated.
Keyphrases
  • magnetic resonance
  • high resolution
  • dna methylation
  • gene expression
  • solid state
  • mass spectrometry
  • anaerobic digestion
  • atomic force microscopy
  • high speed