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Non-invasive diagnosis of viability in seeds and lichens by infrared thermography under controlled environmental conditions.

Beatriz Fernández-MarínOthmar BuchnerGerald KastbergerFederica PiombinoJosé Ignacio García-PlazaolaIlse Kranner
Published in: Plant methods (2019)
Infrared thermography appears to be a promising method for the diagnosis of viability of desiccation-tolerant tissues at early stages of water uptake. For seeds, it is possible to diagnose viability within the first hours of rehydration, after which time they can still be re-dried and stored until further use. We envisage our work as a baseline study for the use of IR imaging techniques to investigate physiological heterogeneity of desiccation tolerant life forms such as lichens, which can be used for biomonitoring, and for sorting live and dead seeds, which is potentially useful for the seed trade.
Keyphrases
  • high resolution
  • gene expression
  • single cell
  • room temperature
  • mass spectrometry
  • photodynamic therapy