Mycobacterium abscessus extracellular vesicles increase mycobacterial resistance to clarithromycin in vitro.
Charlie A VermeireXuejuan TanYurong LiangStephen K KoteyJanet RogersSteven D HartsonLin LiuYong ChengPublished in: Proteomics (2024)
Nontuberculous Mycobacteria (NTM) are a group of emerging bacterial pathogens that have been identified in cystic fibrosis (CF) patients with microbial lung infections. The treatment of NTM infection in CF patients is challenging due to the natural resistance of NTM species to many antibiotics. Mycobacterium abscessus is one of the most common NTM species found in the airways of CF patients. In this study, we characterized the extracellular vesicles (EVs) released by drug-sensitive M. abscessus untreated or treated with clarithromycin (CLR), one of the frontline anti-NTM drugs. Our data show that exposure to CLR increases mycobacterial protein trafficking into EVs as well as the secretion of EVs in culture. Additionally, EVs released by CLR-treated M. abscessus increase M. abscessus resistance to CLR when compared to EVs from untreated M. abscessus. Proteomic analysis further indicates that EVs released by CLR-treated M. abscessus carry an increased level of 50S ribosomal subunits, the target of CLR. Taken together, our results suggest that EVs play an important role in M. abscessus resistance to CLR treatment.
Keyphrases
- cystic fibrosis
- newly diagnosed
- end stage renal disease
- mycobacterium tuberculosis
- ejection fraction
- chronic kidney disease
- pseudomonas aeruginosa
- helicobacter pylori
- prognostic factors
- emergency department
- machine learning
- helicobacter pylori infection
- patient reported outcomes
- small molecule
- microbial community
- deep learning
- air pollution
- amino acid
- drug induced
- antimicrobial resistance