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High-contrast en bloc staining of mouse whole-brain and human brain samples for EM-based connectomics.

Kun SongZhihui FengMoritz Helmstaedter
Published in: Nature methods (2023)
Connectomes of human cortical gray matter require high-contrast homogeneously stained samples sized at least 2 mm on a side, and a mouse whole-brain connectome requires samples sized at least 5-10 mm on a side. Here we report en bloc staining and embedding protocols for these and other applications, removing a key obstacle for connectomic analyses at the mammalian whole-brain level.
Keyphrases
  • resting state
  • white matter
  • functional connectivity
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  • contrast enhanced
  • brain injury
  • computed tomography
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