A fission yeast cell-based system for multidrug resistant HIV-1 proteases.
Zsigmond BenkoDong LiangGe LiRobert T ElderAnindya SarkarJun TakayamaArun K GhoshRichard Y ZhaoPublished in: Cell & bioscience (2017)
The three clinically isolated mdrPRs maintained their viral proteolytic activities and drug resistance in the fission yeast. Furthermore, those viral mdrPR activities were coupled with the induction of growth inhibition and cell death, which could be used to test the PI activities. Indeed, the five investigational PIs and DRV suppressed the wtPR in fission yeast as they did in mammalian cells. Significantly, two of the high level mdrPRs (M10PR and M11PR) were resistant to all of the existing PI drugs including DRV. This observation underscores the importance of continued searching for new PIs against mdrPRs.
Keyphrases
- cell death
- multidrug resistant
- saccharomyces cerevisiae
- sars cov
- human immunodeficiency virus
- antiretroviral therapy
- hiv infected
- hepatitis c virus
- cell wall
- hiv positive
- single cell
- hiv aids
- hiv testing
- drug resistant
- gram negative
- clinical trial
- pseudomonas aeruginosa
- bone marrow
- cell proliferation
- cell cycle arrest
- men who have sex with men
- cystic fibrosis
- south africa
- double blind