Oxazole-Containing Diterpenoids from Cell Cultures of Salvia miltiorrhiza and Their Anti-HIV-1 Activities.
De-Wu ZhangJiamei GuoMin ZhangXiao LiuMingyu BaXiaoyu TaoLi-Yan YuYing GuoJun-Gui DaiPublished in: Journal of natural products (2017)
Four new oxazole-containing diterpenoids, salvianans A-D (1-4), along with three known diterpenoids (5-7), were isolated from Salvia miltiorrhiza cell cultures. The structures of the new compounds were elucidated using spectroscopic methods and single-crystal X-ray diffraction. The evaluation for their anti-HIV-1 activities revealed that 2 and 3 displayed inhibitory activities with IC50 values of 0.03 and 1.2 μM, respectively. The time of addition (TOA) assay and long terminal repeat (LTR) luciferase reporter assay results indicated that compound 2 was an HIV-1 transcription inhibitor and might be a lead compound of antiviral agents acting on HIV-1 transcription.
Keyphrases
- antiretroviral therapy
- hiv positive
- hiv infected
- hiv testing
- human immunodeficiency virus
- hiv aids
- hepatitis c virus
- men who have sex with men
- single cell
- high resolution
- high throughput
- south africa
- cell therapy
- transcription factor
- stem cells
- computed tomography
- magnetic resonance
- molecular docking
- mesenchymal stem cells
- molecular dynamics simulations
- clinical evaluation