Tailoring zirconia surface topography via femtosecond laser-induced nanoscale features: effects on osteoblast cells and antibacterial properties.
Arash GhalandarzadehMonireh GanjaliMilad HosseiniPublished in: Biomedical materials (Bristol, England) (2024)
The performance and long-term durability of dental implants hinge on the quality of bone integration and their resistance to bacteria. This research aims to introduce a surface modification strategy for zirconia implants utilizing femtosecond laser ablation techniques, exploring their impact on osteoblast cell behavior and bacterial performance, as well as the integral factors influencing the soft tissue quality surrounding dental implants. Ultrafast lasers were employed to craft nanoscale groove geometries on zirconia surfaces, with thorough analyses conducted using x-ray diffraction, scanning electron microscopy, atomic force microscopy, and water contact angle measurements. The study evaluated the response of human fetal osteoblastic cell lines to textured zirconia ceramics by assessing alkaline phosphatase activity, collagen I, and interleukin 1 β secretion over a 7 day period. Additionally, the antibacterial behavior of the textured surfaces was investigated using Fusobacterium nucleatum , a common culprit in infections associated with dental implants. Ciprofloxacin (CIP), a widely used antibacterial antibiotic, was loaded onto zirconia ceramic surfaces. The results of this study unveiled a substantial reduction in bacterial adhesion on textured zirconia surfaces. The fine biocompatibility of these surfaces was confirmed through the MTT assay and observations of cell morphology. Moreover, the human fetal osteoblastic cell line exhibited extensive spreading and secreted elevated levels of collagen I and interleukin 1 β in the modified samples. Drug release evaluations demonstrated sustained CIP release through a diffusion mechanism, showcasing excellent antibacterial activity against pathogenic bacteria, including Streptococcus mutans, Pseudomonas aeruginosa , and Escherichia coli .
Keyphrases
- biofilm formation
- pseudomonas aeruginosa
- atomic force microscopy
- electron microscopy
- escherichia coli
- soft tissue
- staphylococcus aureus
- candida albicans
- cystic fibrosis
- drug release
- endothelial cells
- wound healing
- high speed
- drug delivery
- silver nanoparticles
- high resolution
- single cell
- induced apoptosis
- air pollution
- high glucose
- vascular smooth muscle cells
- mesenchymal stem cells
- mass spectrometry
- computed tomography
- body composition
- cancer therapy
- drug induced
- stem cells
- cell proliferation
- diabetic rats
- bone mineral density
- cell cycle arrest
- dual energy
- endoplasmic reticulum stress
- signaling pathway
- magnetic resonance