Generation of Functional Cardiomyocytes from Human Gastric Fibroblast-Derived Induced Pluripotent Stem Cells.
Chih-Hsien WuHsuan-Hwai LinYi-Ying WuYi-Lin ChiuLi-Yen HuangCheng-Chung ChengChung-Chi YangTsung-Neng TsaiPublished in: Biomedicines (2021)
Coronary artery diseases are major problems of the world. Coronary artery disease patients frequently suffer from peptic ulcers when they receive aspirin treatment. For diagnostic and therapeutic purposes, the implementation of panendoscopy (PES) with biopsy is necessary. Some biopsy samples are wasted after the assay is completed. In the present study, we established a protocol for human gastric fibroblast isolation and induced pluripotent stem cell (iPSC) generation from gastric fibroblasts via PES with biopsy. We showed that these iPSCs can be differentiated into functional cardiomyocytes in vitro. To our knowledge, this is the first study to generate iPSCs from gastric fibroblasts in vitro.
Keyphrases
- induced pluripotent stem cells
- coronary artery disease
- stem cells
- endothelial cells
- coronary artery
- high glucose
- end stage renal disease
- low dose
- primary care
- ultrasound guided
- chronic kidney disease
- peritoneal dialysis
- type diabetes
- pulmonary artery
- prognostic factors
- high throughput
- extracellular matrix
- heart failure
- oxidative stress
- bone marrow
- single cell
- drug induced