Phenotypic and transcriptional changes in peripheral blood mononuclear cells during alphavirus encephalitis in mice.
Benjamin H NguyenMaggie L BartlettElizabeth M TroisiElise StanleyDiane E GriffinPublished in: mBio (2024)
The outcome of viral encephalomyelitis is dependent on the host immune response, with clearance and resolution of infection mediated by the adaptive immune response. These processes are frequently studied in mouse models of infection, where infected tissues are examined to understand the mechanisms of clearance and recovery. However, studies of human infection typically focus on the analysis of cells from the blood, a compartment rarely examined in mice, rather than inaccessible tissue. To close this gap, we used single-cell RNA sequencing and flow cytometry to profile the transcriptomic and phenotypic changes of peripheral blood mononuclear cells (PBMCs) before and after central nervous system (CNS) infection in mice. Changes to T and B cell gene expression and cell composition occurred in PBMC and during entry into the CNS, with CCL5 being a differentially expressed chemokine. Therefore, dynamic changes occur in the blood as well as the CNS during the response of mice to virus infection, which will inform the analysis of human studies.
Keyphrases
- single cell
- gene expression
- immune response
- high fat diet induced
- endothelial cells
- rna seq
- flow cytometry
- blood brain barrier
- high throughput
- dna methylation
- type diabetes
- wild type
- dendritic cells
- mouse model
- adipose tissue
- stem cells
- insulin resistance
- toll like receptor
- oxidative stress
- single molecule
- inflammatory response
- liver injury
- drug induced
- solid state
- heat shock