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Rapid and low-cost amplicon visualization for nucleic acid amplification tests using magnetic microbeads.

Michihiko NakanoMasafumi InabaJunya Suehiro
Published in: The Analyst (2021)
This study presents a rapid and low-cost amplicon detection method in which amplicons are attached to magnetic microbeads, suspended in deionized water, and subjected to a magnetic field on a hydrophilic surface resulting in the circular agglomeration of amplicon-conjugated microbeads, visible to the naked eye.
Keyphrases
  • low cost
  • nucleic acid
  • loop mediated isothermal amplification
  • molecularly imprinted
  • sensitive detection
  • label free
  • liquid chromatography
  • solid phase extraction
  • mass spectrometry
  • quantum dots