Competition-based, quantitative chemical proteomics in breast cancer cells identifies new target profiles for sulforaphane.

James A ClulowElisabeth M StorckThomas Lanyon-Hogg Karunakaran A Kalesh Lyn H JonesEdward W Tate

Published in: Chemical communications (Cambridge, England) (2018)

Sulforaphane is a small molecule isothiocyanate which exhibits anticancer potential, yet its biological targets remain poorly understood. Here we employ a competition-based chemical proteomics strategy to profile sulforaphane's targets and identify over 500 targets along with their relative affinities. These targets provide a new set of mediators for sulforaphane's bioactivity, and aid understanding of its complex mode of action.

Keyphrases

small molecule
mass spectrometry
breast cancer cells
gene expression
high resolution
climate change