Intravital imaging of orthotopic and ectopic bone.
Kunihiko HashimotoTakashi KaitoJunichi KikutaMasaru IshiiPublished in: Inflammation and regeneration (2020)
Bone homeostasis is dynamically regulated by a balance between bone resorption by osteoclasts and bone formation by osteoblasts. Visualizing and evaluating the dynamics of bone cells in vivo remain difficult using conventional technologies, including histomorphometry and imaging analysis. Over the past two decades, multiphoton microscopy, which can penetrate thick specimens, has been utilized in the field of biological imaging. Using this innovative technique, the in vivo dynamic motion of bone metabolism-related cells and their interactions has been revealed. In this review, we summarize previous approaches used for bone imaging and provide an overview of current bone tissue imaging methods using multiphoton excitation microscopy.
Keyphrases
- high resolution
- bone mineral density
- bone loss
- soft tissue
- bone regeneration
- induced apoptosis
- postmenopausal women
- high speed
- cell cycle arrest
- optical coherence tomography
- body composition
- single molecule
- oxidative stress
- mass spectrometry
- fluorescence imaging
- endoplasmic reticulum stress
- cell death
- signaling pathway
- drug induced