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Mapping of Major Fusarium Head Blight Resistance from Canadian Wheat cv. AAC Tenacious.

Raman DhariwalMaria A HenriquezColin HiebertCurt A McCartneyHarpinder S Randhawa
Published in: International journal of molecular sciences (2020)
Fusarium head blight (FHB) is one of the most devastating wheat disease due to its direct detrimental effects on grain-yield, quality and marketability. Resistant cultivars offer the most effective approach to manage FHB; however, the lack of different resistance resources is still a major bottleneck for wheat breeding programs. To identify and dissect FHB resistance, a doubled haploid wheat population produced from the Canadian spring wheat cvs AAC Innova and AAC Tenacious was phenotyped for FHB response variables incidence and severity, visual rating index (VRI), deoxynivalenol (DON) content, and agronomic traits days to anthesis (DTA) and plant height (PHT), followed by single nucleotide polymorphism (SNP) and simple sequence repeat (SSR) marker genotyping. A high-density map was constructed consisting of 10,328 markers, mapped on all 21 chromosomes with a map density of 0.35 cM/marker. Together, two major quantitative trait loci for FHB resistance were identified on chromosome 2D from AAC Tenacious; one of these loci on 2DS also colocated with loci for DTA and PHT. Another major locus for PHT, which cosegregates with locus for low DON, was also identified along with many minor and epistatic loci. QTL identified from AAC Tenacious may be useful to pyramid FHB resistance.
Keyphrases
  • high density
  • genome wide
  • genome wide association study
  • dna methylation
  • high resolution
  • copy number
  • public health
  • genome wide association
  • risk factors
  • mass spectrometry
  • optical coherence tomography
  • single cell