N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics.
Tin-Yan KooHinyuk LaiDaniel K NomuraClive Yik-Sham ChungPublished in: Nature communications (2023)
Cysteine has been exploited as the binding site of covalent drugs. Its high sensitivity to oxidation is also important for regulating cellular processes. To identify new ligandable cysteines which can be hotspots for therapy and to better study cysteine oxidations, we develop cysteine-reactive probes, N-acryloylindole-alkynes (NAIAs), which have superior cysteine reactivity owing to delocalization of π electrons of the acrylamide warhead over the whole indole scaffold. This allows NAIAs to probe functional cysteines more effectively than conventional iodoacetamide-alkyne, and to image oxidized thiols by confocal fluorescence microscopy. In mass spectrometry experiments, NAIAs successfully capture new oxidized cysteines, as well as a new pool of ligandable cysteines and proteins. Competitive activity-based protein profiling experiments further demonstrate the ability of NAIA to discover lead compounds targeting these cysteines and proteins. We show the development of NAIAs with activated acrylamide for advancing proteome-wide profiling and imaging ligandable cysteines and oxidized thiols.
Keyphrases
- living cells
- high resolution
- fluorescent probe
- single molecule
- low density lipoprotein
- mass spectrometry
- single cell
- stem cells
- small molecule
- deep learning
- high throughput
- optical coherence tomography
- liquid chromatography
- hydrogen peroxide
- binding protein
- photodynamic therapy
- smoking cessation
- high performance liquid chromatography
- protein protein