Use of Electron Paramagnetic Resonance (EPR) to Evaluate Redox Status in a Preclinical Model of Acute Lung Injury.
Hanan B ElajailiNathan M DeeSergey I DikalovJoseph P Y KaoEva S NozikPublished in: Molecular imaging and biology (2023)
We have developed protocols for delivering EPR spin probes in vivo, allowing detection of cellular and mitochondrial superoxide in lung injury by EPR. Superoxide measurements by EPR could differentiate mice with and without lung injury, as well as mouse strains with different disease susceptibilities. We expect these protocols to capture real-time superoxide production and enable evaluation of lung EPR imaging as a potential clinical tool for subphenotyping ARDS patients based on redox status.
Keyphrases
- hydrogen peroxide
- end stage renal disease
- ejection fraction
- newly diagnosed
- chronic kidney disease
- single molecule
- lipopolysaccharide induced
- high resolution
- escherichia coli
- acute respiratory distress syndrome
- electron transfer
- prognostic factors
- adipose tissue
- cell therapy
- human health
- room temperature
- living cells
- nucleic acid
- real time pcr