Treatment of NSAPs-rich petrochemical wastewaters using a two-stage combined process of fungi and activated sludge.

A two-stage biological process using fungi and bacteria was set-up and tested for treating a petrochemical wastewater containing naphthalene sulphonic acid polymers. The fungal treatment was carried out through a trickling filter inoculated with Pleurotus ostreatus attached on Luffa cylindrica acting as both physical support and carbon source. The fungal reactor was operated in non-sterile conditions setting two pH values (5 and 6) and two hydraulic retention times (2 d and 3 d). The effluent was then sent to an activated sludge reactor operating the second stage of the treatment. Using an HPLC-based technique, it was observed that the fungal reactor was capable of reducing the polymerization grade of naphthalene sulphonic acid polymers up to 35%, thus increasing significantly the biodegradability of the petrochemical wastewater, from the initial 9% to 46%. The two-stage process allowed to remove about 50% of the total COD much higher than 9% that can be achieved with activated sludge alone. The use of Luffa cylindrica as support for fungi allowed to limit bacterial contamination of the trickling filter and enhanced enzymatic production (on average 20 U/L of Laccase) without any release of non-biodegradable by-products in the effluent. Extraction and PCR-amplification of fungal DNA was carried out along with over 70 d running process in order to monitor the changes of the fungal community inside the reactors. Results showed that Meyerozyma, Fusarium and Thricoderma, spp. developed inside the reactor with Thricoderma, spp. representing the main constituent of fungal biomass at the end of the experiment.