Discovery of an embryonically derived bipotent population of endothelial-macrophage progenitor cells in postnatal aorta.
Anna E WilliamsonSanuri LiyanageMohammadhossein HassanshahiMalathi S I DonaDeborah Toledo-FloresDang X A TranCatherine DimasiNisha SchwarzSanuja FernandoThalia SalagarasAaron LongJan KazenwadelNatasha L HarveyGrant R DrummondAntony VinhVashe ChandrakanthanAshish MisraZoltan NeufeldJoanne T M TanLuciano G MartelottoMohammad Mahfuz ChowdhuryClaudine S BonderAlexander R PintoShiwani SharmaStephen J NichollsChristina A BursillPeter James PsaltisPublished in: Nature communications (2024)
Converging evidence indicates that extra-embryonic yolk sac is the source of both macrophages and endothelial cells in adult mouse tissues. Prevailing views are that these embryonically derived cells are maintained after birth by proliferative self-renewal in their differentiated states. Here we identify clonogenic endothelial-macrophage (EndoMac) progenitor cells in the adventitia of embryonic and postnatal mouse aorta, that are independent of Flt3-mediated bone marrow hematopoiesis and derive from an early embryonic CX 3 CR1 + and CSF1R + source. These bipotent progenitors are proliferative and vasculogenic, contributing to adventitial neovascularization and formation of perfused blood vessels after transfer into ischemic tissue. We establish a regulatory role for angiotensin II, which enhances their clonogenic and differentiation properties and rapidly stimulates their proliferative expansion in vivo. Our findings demonstrate that embryonically derived EndoMac progenitors participate in local vasculogenic responses in the aortic wall by contributing to the expansion of endothelial cells and macrophages postnatally.
Keyphrases
- endothelial cells
- angiotensin ii
- aortic valve
- bone marrow
- pulmonary artery
- vascular endothelial growth factor
- high glucose
- preterm infants
- adipose tissue
- induced apoptosis
- vascular smooth muscle cells
- acute myeloid leukemia
- angiotensin converting enzyme
- small molecule
- mesenchymal stem cells
- left ventricular
- heart failure
- transcription factor
- cell cycle arrest
- pulmonary hypertension
- cell death
- coronary artery
- gestational age
- brain injury
- single cell
- cerebral ischemia
- blood brain barrier
- optical coherence tomography
- hematopoietic stem cell