Single cell, Label free Characterisation of Human Mesenchymal Stromal cell Stemness and Future Growth Potential by Autofluorescence Multispectral Imaging.
Jared M CampbellAbbas HabibalahiAdnan AghaShannon HandleyAline KnabXiaohu XuAkanksha BhargavaZhilin LeiMax MackeviciusYuan TianSaabah B MahbubAyad G AnwerStan GronthosSharon PatonShane T GreyLindsay E WuRobert B GilchristEwa M GoldysPublished in: Stem cell reviews and reports (2024)
This non-invasive methodology enabled the in-depth characterisation of hBM-MSCs from a single assay. This approach is advantageous for clinical applications as well as research and stands out for the characterisation of both present status as well as future behaviour. The use of data from five MSC lines with heterogenous AFMI profiles supports potential generalisability.
Keyphrases
- single cell
- label free
- stem cells
- bone marrow
- high throughput
- current status
- rna seq
- endothelial cells
- mesenchymal stem cells
- high resolution
- epithelial mesenchymal transition
- human health
- fluorescence imaging
- electronic health record
- optical coherence tomography
- big data
- machine learning
- umbilical cord
- artificial intelligence