Application of ethyl cinnamate based optical tissue clearing and expansion microscopy combined with retrograde perfusion for 3D lung imaging.

Quanchao SunTiziana PicasciaArif Ul Maula KhanCinzia BrennaVincent HeuvelineAnja SchmausJonathan P SleemanNorbert Gretz

Published in: Experimental lung research (2020)

The retrograde perfusion combined with trachea ligation technique could be applied in the lung research in mice. 3 D structure of lung vasculature can be visualized by MHI148-PEI perfusion and ECi based OTC in an efficient way. ExM and immunofluorescence staining protocol is highly recommended to perform 3 D imaging of fresh fixed lung as well as paraffin-embedded lung blocks.

Keyphrases

high resolution
magnetic resonance imaging
contrast enhanced
type diabetes
single molecule
computed tomography
high throughput
optical coherence tomography
insulin resistance
high fat diet induced