Generation of Recombinant Antibodies in HEK293F Cells for the Detection of Staphylococcus aureus .
Joo-Kyung KimGyu-Min LimEun-Jung KimWooseong KimChang-Soo LeeByung Gee KimHee-Jin JeongPublished in: ACS omega (2022)
Staphylococcus aureus is a major resistant pathogen in clinical practice. Due to the increasing number of infections, rapid and sensitive detection of antibiotic-resistant S. aureus as well as antibiotic-sensitive S. aureus is important for the prevention and control of infectious diseases. In this study, we produced recombinant antibodies against S. aureus from mammalian human embryonic kidney 293 Freestyle cells with high yield and purity. These recombinant antibodies showed high binding affinity and low detection limit in both indirect and sandwich enzyme-linked immunosorbent assays for the detection of methicillin-resistant S. aureus and methicillin-sensitive S. aureus . These results suggest that the recombinant antibodies produced herein can be used for the accurate detection of S. aureus with a wild range of applications in medical diagnosis, food safety, and drug discovery.
Keyphrases
- staphylococcus aureus
- loop mediated isothermal amplification
- sensitive detection
- induced apoptosis
- drug discovery
- real time pcr
- clinical practice
- infectious diseases
- label free
- cell cycle arrest
- methicillin resistant staphylococcus aureus
- biofilm formation
- endothelial cells
- quantum dots
- high resolution
- cell death
- mass spectrometry
- endoplasmic reticulum stress
- high throughput
- pi k akt
- induced pluripotent stem cells
- capillary electrophoresis