A fluorogenic RNA aptamer nanodevice for the low background imaging of mRNA in living cells.

Tingting XuYao SunSha YuShaojun WuYu SuYe Tian Yuanzhen Zhou Jun-Jie Zhu

Published in: Chemical communications (Cambridge, England) (2022)

A fluorogenic RNA aptamer nanodevice integrating an entropy-driven RNA amplifier with near-infrared (NIR) light control was developed, affording high contrast and sensitivity for imaging low-abundance mRNA in living cells. The design principle offers a new approach for developing low-background imaging systems for live-cell studies and manipulation.

Keyphrases

living cells
fluorescent probe
high resolution
single molecule
gold nanoparticles
sensitive detection
fluorescence imaging
photodynamic therapy
binding protein
drug delivery
case control