Transfection of Unmodified MicroRNA Using Monolayer-Coated Au Nanoparticles as Gene-Delivery Vehicles.
Johnson HoangSagar L PatilPannaree SrinoiTingting LiuMaria D MarquezOrawan KhantamatWirote TuntiwechapikulPreethi H GunaratneT Randall LeePublished in: ACS applied bio materials (2023)
This article describes a monolayer-coated gold nanoparticle-based transfection system for the delivery of microRNA (miRNA) into human osteosarcoma (HOS) cells. Two distinct ammonium-terminated adsorbates were used in this study, which provided a platform for ionic bonding of the miRNA onto gold nanoparticles (AuNPs). The custom-designed monolayer-coated gold nanoparticles were characterized by dynamic light scattering, gel mobility shift assay, transmission electron microscopy, ultraviolet-visible spectrometry, zeta potential, and X-ray photoelectron spectroscopy. The miRNA-loaded gold nanoparticles were transfected, and the level of intracellular miRNA delivered and taken up by cells was measured by Taqman qPCR. The overall analysis indicated a successful delivery of miRNA into the HOS cells at an ∼11,000-fold increase compared to nontreated cells.
Keyphrases
- gold nanoparticles
- induced apoptosis
- cell cycle arrest
- high resolution
- reduced graphene oxide
- endoplasmic reticulum stress
- high throughput
- drug delivery
- magnetic resonance imaging
- endothelial cells
- electron microscopy
- oxidative stress
- cell death
- risk assessment
- climate change
- cell proliferation
- single molecule
- data analysis
- tandem mass spectrometry