Chromatin accessibility of kidney tubular cells under stress reveals key transcription factor mediating acute and chronic kidney disease.
Yuexian XingQi WangJing ZhangWenju LiAiping DuanJingping YangZhihong LiuPublished in: The FEBS journal (2021)
Cellular injury caused by stimuli plays an important role in the progression of various diseases including acute and chronic kidney diseases. The dynamic transcriptional regulation responding to stimuli underlies the important mechanism of injury. In this study, we investigated the regulatory elements and their dynamic activities in kidney tubular epithelial cells. We captured the chromatin accessibility and gene expression with ATAC-seq and RNA sequencing under a variety of extracellular stimuli including H2 O2 , TGF-β1, and FG4592 which is an agonist of hypoxia-inducible factor. Our results revealed both condition-specific and condition-shared transcription regulation. Interestingly, the shared regulation program revealed that the key transcription factor HNF1B-mediated cellular reprogramming leads to a common change among the stimuli. We found the HNF1B regulatory network was significantly disrupted in various kidney diseases.
Keyphrases
- transcription factor
- gene expression
- single cell
- chronic kidney disease
- liver failure
- dna binding
- respiratory failure
- genome wide
- drug induced
- induced apoptosis
- rna seq
- genome wide identification
- dna methylation
- dna damage
- quality improvement
- oxidative stress
- cell cycle arrest
- transforming growth factor
- cell death
- resting state
- stress induced
- functional connectivity
- toll like receptor
- high resolution
- pi k akt