Highly Sensitive Detection of IDH2 Mutations in Acute Myeloid Leukemia.

Jessica PetitiValentina RossoEleonora CroceVanessa FranceschiGiacomo AndreaniMatteo DraganiMarco De GobbiMonia LunghiGiuseppe SaglioCarmen FavaMarco Lo IaconoDaniela Cilloni

Published in: Journal of clinical medicine (2020)

We found that PNA-PCR clamping and digital PCR identified IDH2 mutations in DNA samples with comparable results in a percentage significantly higher compared to Sanger sequencing. PNA-PCR clamping can be used even in laboratories not equipped for sophisticated analyses, decreasing cost and time for IDH2 characterization.

Keyphrases

real time pcr
low grade
nucleic acid
wild type
single molecule
label free
circulating tumor
single cell
cell free
mass spectrometry
fluorescent probe
living cells
molecularly imprinted