Analysis of Ice-Binding Protein Evolution.

Discovering novel ice-binding proteins (IBPs) is important for understanding the evolution of IBPs but it is difficult to determine where resources should be directed in the search for novel IBPs. For this reason, we developed a simple bioinformatic approach for aiding in the determination of where to direct efforts in the search for IBPs. First, BLAST is used to obtain a candidate list of putative IBPs. Next, phylogenetic trees are constructed to map the candidate list of putative IBPs to determine if any patterns are forming. These candidate putative IBPs and their patterns are then assessed through the production of ancestral sequences and reverse BLAST searches, in addition to the use of IBP calculators, to determine which sequences should be cut to produce the final putative IBP list. Finally, we explain an avenue to investigate these putative IBPs further for the development of hypotheses on their evolution.