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Functional integration of a semi-synthetic azido-queuosine derivative into translation and a tRNA modification circuit.

Larissa BesslerNavpreet KaurLea-Marie VogtLaurin FlemmichCarmen SiebenallerMarie-Luise WinzFrancesca TuortoRonald MicuraAnn E Ehrenhofer-MurrayMark Helm
Published in: Nucleic acids research (2022)
Substitution of the queuine nucleobase precursor preQ1 by an azide-containing derivative (azido-propyl-preQ1) led to incorporation of this clickable chemical entity into tRNA via transglycosylation in vitro as well as in vivo in Escherichia coli, Schizosaccharomyces pombe and human cells. The resulting semi-synthetic RNA modification, here termed Q-L1, was present in tRNAs on actively translating ribosomes, indicating functional integration into aminoacylation and recruitment to the ribosome. The azide moiety of Q-L1 facilitates analytics via click conjugation of a fluorescent dye, or of biotin for affinity purification. Combining the latter with RNAseq showed that TGT maintained its native tRNA substrate specificity in S. pombe cells. The semi-synthetic tRNA modification Q-L1 was also functional in tRNA maturation, in effectively replacing the natural queuosine in its stimulation of further modification of tRNAAsp with 5-methylcytosine at position 38 by the tRNA methyltransferase Dnmt2 in S. pombe. This is the first demonstrated in vivo integration of a synthetic moiety into an RNA modification circuit, where one RNA modification stimulates another. In summary, the scarcity of queuosinylation sites in cellular RNA, makes our synthetic q/Q system a 'minimally invasive' system for placement of a non-natural, clickable nucleobase within the total cellular RNA.
Keyphrases
  • escherichia coli
  • minimally invasive
  • nucleic acid
  • induced apoptosis
  • quantum dots
  • gene expression
  • mass spectrometry
  • cell death
  • big data
  • cell cycle arrest
  • oxidative stress