Poly-L-lysine as a crosslinker in bile acid and alginate nanoaggregates for gene delivery in auditory cells.
Thomas FosterCorina Mihaela IonescuMelissa JonesSusbin Raj WagleBožica KovacevicPatrick LimArmin MooranianHani-Al SalamiPublished in: Nanomedicine (London, England) (2023)
Background: Hearing loss is a condition that may affect a wide array of patients from various backgrounds. There are no cures for sensorineural hearing loss. Gene therapy is one possible method of improving hearing status; however, gene delivery remains challenging. Materials & methods: Polymer nanoaggregates of alginate and poly-L-lysine were prepared with and without bile acid. The nanoaggregates had physical properties, cytotoxicity, gene release and gene expression analyzed. Results & discussion: The nanoparticles produced had appropriate size and charge, low cytotoxicity between 0.5 and 1.0 mg/ml and linear gene release but poor transfection efficiency. Conclusion: The present study provides preliminary evidence for the efficacy of polymer nanotechnology with bile acids for inner ear gene delivery; optimization is required to improve transfection efficiency.
Keyphrases
- hearing loss
- gene expression
- gene therapy
- end stage renal disease
- induced apoptosis
- genome wide
- ejection fraction
- newly diagnosed
- chronic kidney disease
- copy number
- dna methylation
- physical activity
- prognostic factors
- peritoneal dialysis
- mental health
- high resolution
- high throughput
- oxidative stress
- genome wide identification
- cell proliferation
- signaling pathway
- amino acid
- patient reported outcomes
- endoplasmic reticulum stress
- mass spectrometry
- tissue engineering
- high density
- pi k akt
- neural network