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Reference genes for mesangial cell and podocyte qPCR gene expression studies under high-glucose and renin-angiotensin-system blocker conditions.

Nicole Dittrich HosniAna Carolina AnauateMirian Aparecida Boim
Published in: PloS one (2021)
This analysis provides support for the use of HPRT and ACTB as reference genes in mouse mesangial cell studies of gene expression via real-time PCR, while for podocytes, HPRT and GAPDH should be chosen.
Keyphrases
  • high glucose
  • gene expression
  • endothelial cells
  • diabetic nephropathy
  • single cell
  • dna methylation
  • real time pcr
  • genome wide
  • cell therapy
  • stem cells
  • transcription factor
  • mesenchymal stem cells
  • bone marrow