Si-rhodamine fluorescent probe for monitoring of hypochlorous acid in the brains of mice afflicted with neuroinflammation.
Fanghui LiangJian JiangXinyue YangGuoyang ZhangJiaying ZhouJiahao HanYujie GengZhuo WangPublished in: Chemical communications (Cambridge, England) (2023)
Neuroinflammation leads to a persistent oxidative stress in the brain, and is closely related to the pathology of various neurological disorders. Hypochlorous acid (HClO) is a reactive oxygen species (ROS) that, at high levels, can cause brain tissue damage and neurogenic apoptosis. Herein, we designed and synthesized a silicon-rhodamine (SiR)-based formohydrazide (FH)-containing fluorescent probe, denoted as SiR-FH, for sensing HClO. This probe showed good selectivity, rapid response and high sensitivity. SiR-FH was successfully used to detect endogenous and exogenous HClO in living cells. Moreover, SiR-FH realized real-time monitoring of change in HClO flux in the brains of mice with LPS-induced neuroinflammation. The probe provides a practical tool for the monitoring of oxidative stress related to neuroinflammation.
Keyphrases
- fluorescent probe
- living cells
- lps induced
- oxidative stress
- inflammatory response
- reactive oxygen species
- cerebral ischemia
- dna damage
- lipopolysaccharide induced
- high fat diet induced
- resting state
- traumatic brain injury
- diabetic rats
- white matter
- cell death
- ischemia reperfusion injury
- induced apoptosis
- spinal cord injury
- subarachnoid hemorrhage
- cognitive impairment
- metabolic syndrome
- insulin resistance
- signaling pathway
- functional connectivity
- endoplasmic reticulum stress
- blood brain barrier
- multiple sclerosis
- skeletal muscle
- cell cycle arrest
- quantum dots
- drug induced