Paper-Based Antibody Detection Devices Using Bioluminescent BRET-Switching Sensor Proteins.
Keisuke TendaBenice van GervenRemco ArtsYuki TokuraMaarten MerkxDaniel CitterioPublished in: Angewandte Chemie (International ed. in English) (2018)
This work reports on fully integrated "sample-in-signal-out" microfluidic paper-based analytical devices (μPADs) relying on bioluminescence resonance energy transfer (BRET) switches for analyte recognition and colorimetric signal generation. The devices use BRET-based antibody sensing proteins integrated into vertically assembled layers of functionalized paper, and their design enables sample volume-independent and fully reagent-free operation, including on-device blood plasma separation. User operation is limited to the application of a single drop (20-30 μL) of sample (serum, whole blood) and the acquisition of a photograph 20 min after sample introduction, with no requirement for precise pipetting, liquid handling, or analytical equipment except for a camera. Simultaneous detection of three different antibodies (anti-HIV1, anti-HA, and anti-DEN1) in whole blood was achieved. Given its simplicity, this type of device is ideally suited for user-friendly point-of-care testing in low-resource environments.
Keyphrases
- energy transfer
- quantum dots
- label free
- liquid chromatography
- gold nanoparticles
- loop mediated isothermal amplification
- antiretroviral therapy
- hiv positive
- hepatitis c virus
- sensitive detection
- human immunodeficiency virus
- hydrogen peroxide
- mass spectrometry
- single cell
- hiv aids
- real time pcr
- high resolution
- ionic liquid
- high speed
- nitric oxide
- convolutional neural network
- living cells
- men who have sex with men
- drug induced
- solar cells
- solid phase extraction